Welcome!
ALERT ATTENTION ⚠️
In observance of the holiday, Juneteenth, Agilent CrossLab/iLab Operations Software Support Help Desk will be closed during U.S. hours on Thursday, June 19th, 2025. We will resume regular U.S. support hours on Friday, June 20th, 2025. EU and APAC Support will remain open during this time. For urgent matters, please add "Urgent" to the ticket/email subject or press "1" when prompted to escalate a call on the iLab Support phone, and we will prioritize those requests first.
The Microscopy Shared Resources provides ready access to up-to-date instrumentation, each instrument with multiple capabilities, to meet the diverse type of samples that need to be examined by Researchers in their current work and as their projects evolve. Fluorescence microscopy for imaging genetically encoded fluorescent proteins constitute the biggest category of instruments. The AxioImager Z1 is an upright fluorescent microscopes is available for standard IF labeling on glass slides. Two LSM spot scan confocals are suited best for imaging thick samples on fixed tissue samples.The Zeiss LSM780 spot scanning confocal microscope to instrument has the most sensitive detection system (GAsP) with photon counting capabilities.The Perkin Elmer spinning disk confocal is especially suited for long term time lapse imaging of cancer cells and FRAP analysis and it also has bright field DIC optics. The Zeiss Observer is an inverted microscope that is configured for bright field color microscopy for imaging paraffin sections (H&E) from mouse cancer models. The Zeiss Observer also has phase contrast, DIC optics and widefield fluorescence with live cell imaging in all those imaging modes. For live imaging of fluorescently encoded cells, the two General Electric DeltaVision microscopes have deconvolution capability. The Microscopy Shared Resource also has a dedicated super-resolution microscope that reaches resolutions of 100nm. Beyond that resolution, Cancer researchers submit samples for various types of transmission electron microscopy examination with the Hitach HT7000 (plastic embedding, thin sectioning, immunogold labeling, negative staining). The transmission electron microscopy service is typically completed entirely by the lab staff with digital images uploaded to shared servers. With the exception of the OMX microscope, all the other instruments are operated by both students, post-docs and Investigators with training and daily assistance for the lab staff. The Microscopy Shared Resource provides training on software for data image analysis that are available on 2 workstations and remotely by a license server.
Erika Tse-Luen Wee, Director
516-367-5079
David Spector, Faculty Head
| Hours | Location |
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9-5 Monday-Friday Open 24/7 for trained users |
Hershey Building (Main entrance -Room 130) James Building (James 101, 104)
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Links and Resources
