Flow Cytometry

Overview of Services

A variety of applications may be used in flow cytometry which include analysis of cell cycle progression, apoptosis, and quantitation of cell surface-expressed receptors, intracellular cytokines, phagocytosis, and enrichment of stem cells from different tissues based on cell-surface markers or subpopulations of cancer cells based on aneuploidy.

 

 This site provides information about specific equipment, services, fees, training, methods, and resources. Facility staff oversees equipment maintenance, trains new users on operation of equipment and is available to assist with assay development. Tissue culture facilities are available for maintenance of cells during experiements.

 

 The Flow Cytometry facility is kept locked for security purposes, but is accessible 24/7 to all users who have been trained on the instrumentation and approved by the facility director.

Instrumentation

  • Becton Dickinson Dual Fortessa Cell Analyzer (Side 1 Nickname: BUG and Side 2 Nickname: FIN)
  • Becton Dickinson FACS ARIA-SORP 1 Cell Sorter (Nickname: Montauk)
  • Becton Dickinson FACS ARIA-SORP 2 Cell Sorter (Nickname: Orient)
  • SONY SH800 Cell Sorter (Nickname: Hunter)
  • SONY ID7000 Spectral Analyzer (Nickname: Rocky)

Leadership

 Lingbo Zhang, Scientific Head

 lbzhang@cshl.edu

 

Pamela Moody, Director

moody@cshl.edu

516.367.8477 (facility) or 5213 (office)

 

Kris Anderson, Resource Technologist I 

kanderso@cshl.edu

516.367.8477 (facility) or 5211 (office)

 

Amanda Jensen, Resource Technologist I (In Training)

jensen@cshl.edu

516.367.8477 (facility) or 5211 (office)

 

Location and hours of operation

   

Hershey Building #22, Room 140

Monday- Friday from 7:00am to 5:00pm

Open 24/7 for trained users      

 

 

 

 

Links and Resources

 

Instrument Manufacturers

  1. BD Biosciences 
  2. Beckman Coulter
  3. Miltenyi
  4. SONY Biotechnology
  5. Thermofisher

 Reference Tables

  1. BD Fluorochrome Specifications Table
  2. BD Fluorescensce Spectrum Viewer 
  3. Conjugation of Monoclonal Antibodies
  4. Compensation of Fluorochrome