Overview of Services
The Engineered Mouse Model core facility assists with the construction and management of genetically altered mouse strains. The primary services provided by this core facility are the generation of transgenic or chimeric mice. Cryopreservation of both mouse embryos and sperm is offered for the storage and management of transgenic mouse lines. In addition to offering these standard services, the facility assists investigators in experiments that require sophisticated embryo manipulation or cell/embryo microinjection techniques.
Leadership
Peter Westcott, Ph.D.westcott@cshl.edu
Yu-Ting Yang, Ph.D.yangy@cshl.edu
Location and hours of operation
Hours Location
Monday - Friday - 9-5
Genome Center
Links and Resources
Mouse Gene Targeting Library The International Gene Trap Consortium (IGTC) CSHL Gene Targeting Site
Search available services:
View: by category
alphabetically
Name
Description
Price
Cryopreserved sample storage cost (line/year)
Annual storage $50 per line
Inquire
DR4 MEF Feeder Cells, Irradiated
DR4 MEF Feeder Cells, Irradiated (1*10^6 / vial)
Inquire
Embryo cryopreservation_Tg x Tg
Cryopreservation of a minimum of 150 embryos from homozygous males or 200 embryos from heterozygous males
Test thaw one embryo straw and evaluate the viability of the embryos
Includes the first year of storage
Annual storage $50 per line
Shipping service for frozen embryos is available upon request; additional processing and shipping fees will apply.
Inquire
Embryo cryopreservation_Tg x WT
Cryopreservation of a minimum of 150 embryos from homozygous males or 200 embryos from heterozygous males
Test thaw one embryo straw and evaluate the viability of the embryos
Includes the first year of storage
Annual storage $50 per line
Shipping service for frozen embryos is available upon request; additional processing and shipping fees will apply.
CSHL CC
$1,622.00
each
CSHL
$1,703.00
each
Embryo cryopreservation_Tg x WT (IVF)
Cryopreservation of a minimum of 150 embryos from homozygous males or 200 embryos from heterozygous males
Test thaw one embryo straw and evaluate the viability of the embryos
Includes the first year of storage
Annual storage $50 per line
Shipping service for frozen embryos is available upon request; additional processing and shipping fees will apply.
Inquire
ES cell derivation (24 blastocysts)
EMM will start with up to 24 blastocysts per session.
Inquire
ES cell derivation (48 blastocysts)
EMM will start with up to 48 blastocysts per session.
Inquire
ES cell targeting
Targeting vector electroporation
C57BL/6 ES cells/drug selection/picking colonies, up to 96 ES clones provided to the investigator for confirming HR
Inquire
Expanding targeted ES cell clones for Cryostorage (per clone)
Inquire
Expanding targeted ES cell clones for injection (per clone)
Inquire
In vitro fertilization (IVF) with cryopreserved sperms
(1)Frozen sperm imported from other institutes are received and stored by the EMM core, or (2)Frozen sperm from the prior CSHL Gene Targeting Core Wild-type oocyte donors (10 females) are purchased from a certified vendor IVF using thawed sperm and wild-type oocytes Surgical transfer of IVF-generated 2-cell embryos into recipient females All litters will be pathogen tested (LAR, additional fees will apply) All litters will be transferred to the requested PI upon receipt of pathogen testing results.
Inquire
In vitro fertilization (IVF) with fresh sperms
IVF using fresh sperm of the investigator's choice and wild-type oocytes Wild-type oocyte donors (10 females) are purchased from a certified vendor Surgical transfer of IVF-generated 2-cell embryos into recipient females
All litters will be pathogen tested (LAR, additional fees will apply) All litters will be transferred to the requested PI upon receipt of pathogen testing results.
Inquire
Oviduct transfer
Transfer up to 20 embryos to oviducts of 1 recipient
Inquire
Sperm cryopreservation_IVF QC
Sperms collected from up to 2 males per session, with 18 to 20 straws stored IVF quality control using oocytes from three wild-type (WT) donor females to produce two-cell embryos Includes the first year of storage Annual storage $50 per line Shipping service for frozen sperm is available upon request; additional processing and shipping fees will apply
Inquire
Sperm cryopreservation_standard QC
Sperms collected from up to 2 males per session, with 18 to 20 straws stored. Includes QC thawing of one straw to evaluate sperm quality. Includes the first year of storage Annual storage $50 per line Shipping service for frozen sperm is available upon request; additional processing and shipping fees will apply
Inquire
SPF Rederivation
Collect embryos from up to 10 donor females per session and transfer the embryos up to 3 SPF recipients.
Inquire
Uterus transfer
Transfer up to 20 embryos to uteri of 1 recipient
Inquire
▼
►
Cryopreservation (6)
Name
Description
Price
Sperm cryopreservation_standard QC
Sperms collected from up to 2 males per session, with 18 to 20 straws stored. Includes QC thawing of one straw to evaluate sperm quality. Includes the first year of storage Annual storage $50 per line Shipping service for frozen sperm is available upon request; additional processing and shipping fees will apply
Inquire
Sperm cryopreservation_IVF QC
Sperms collected from up to 2 males per session, with 18 to 20 straws stored IVF quality control using oocytes from three wild-type (WT) donor females to produce two-cell embryos Includes the first year of storage Annual storage $50 per line Shipping service for frozen sperm is available upon request; additional processing and shipping fees will apply
Inquire
Embryo cryopreservation_Tg x Tg
Cryopreservation of a minimum of 150 embryos from homozygous males or 200 embryos from heterozygous males
Test thaw one embryo straw and evaluate the viability of the embryos
Includes the first year of storage
Annual storage $50 per line
Shipping service for frozen embryos is available upon request; additional processing and shipping fees will apply.
Inquire
Embryo cryopreservation_Tg x WT
Cryopreservation of a minimum of 150 embryos from homozygous males or 200 embryos from heterozygous males
Test thaw one embryo straw and evaluate the viability of the embryos
Includes the first year of storage
Annual storage $50 per line
Shipping service for frozen embryos is available upon request; additional processing and shipping fees will apply.
CSHL CC
$1,622.00
each
CSHL
$1,703.00
each
Embryo cryopreservation_Tg x WT (IVF)
Cryopreservation of a minimum of 150 embryos from homozygous males or 200 embryos from heterozygous males
Test thaw one embryo straw and evaluate the viability of the embryos
Includes the first year of storage
Annual storage $50 per line
Shipping service for frozen embryos is available upon request; additional processing and shipping fees will apply.
Inquire
Cryopreserved sample storage cost (line/year)
Annual storage $50 per line
Inquire
▼
►
IVF (2)
Name
Description
Price
In vitro fertilization (IVF) with cryopreserved sperms
(1)Frozen sperm imported from other institutes are received and stored by the EMM core, or (2)Frozen sperm from the prior CSHL Gene Targeting Core Wild-type oocyte donors (10 females) are purchased from a certified vendor IVF using thawed sperm and wild-type oocytes Surgical transfer of IVF-generated 2-cell embryos into recipient females All litters will be pathogen tested (LAR, additional fees will apply) All litters will be transferred to the requested PI upon receipt of pathogen testing results.
Inquire
In vitro fertilization (IVF) with fresh sperms
IVF using fresh sperm of the investigator's choice and wild-type oocytes Wild-type oocyte donors (10 females) are purchased from a certified vendor Surgical transfer of IVF-generated 2-cell embryos into recipient females
All litters will be pathogen tested (LAR, additional fees will apply) All litters will be transferred to the requested PI upon receipt of pathogen testing results.
Inquire
▼
►
Targeting Services (6)
Name
Description
Price
ES cell derivation (24 blastocysts)
EMM will start with up to 24 blastocysts per session.
Inquire
ES cell derivation (48 blastocysts)
EMM will start with up to 48 blastocysts per session.
Inquire
Expanding targeted ES cell clones for Cryostorage (per clone)
Inquire
Expanding targeted ES cell clones for injection (per clone)
Inquire
ES cell targeting
Targeting vector electroporation
C57BL/6 ES cells/drug selection/picking colonies, up to 96 ES clones provided to the investigator for confirming HR
Inquire
DR4 MEF Feeder Cells, Irradiated
DR4 MEF Feeder Cells, Irradiated (1*10^6 / vial)
Inquire
▼
►
Transfer Services (3)
Name
Description
Price
Oviduct transfer
Transfer up to 20 embryos to oviducts of 1 recipient
Inquire
SPF Rederivation
Collect embryos from up to 10 donor females per session and transfer the embryos up to 3 SPF recipients.
Inquire
Uterus transfer
Transfer up to 20 embryos to uteri of 1 recipient
Inquire
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