Welcome!
The Gene Targeting Shared Resource assists with the construction and management of genetically altered mouse strains. The primary services provided by this shared resource are the generation of transgenic or chimeric mice. Cryopreservation of both mouse embryos and sperm is offered for storage and management of transgenic mouse lines. In addition to offering these standard services, the facility assists investigators in experiments that require sophisticated embryo manipulation or cell/embryo microinjection techniques.
Alea A. Mills Ph.D
mills@cshl.edu,
phone (516) 367-6910,
fax (516) 367-8874
Dr. Leyi Li
lli@cshl.edu
Director Daily operation
516-422-4085
| Hours | Location |
|
Monday - Friday - 8-4:30 |
Genome Center |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Dr. Leyi Li |
Core Director
|
516-422-4085
|
lli@cshl.edu
|
Woodbury Room 203
|
| Jie Ouyang |
Technician I
|
516-422-4072
|
jouyang@cshl.edu
|
Woodbury Room 206
|
| Services |
| ► Cryopreservation (4) | |||
| Name | Description | Price | |
|---|---|---|---|
| Cryopreserved embryo recovery and culture |
One straw thawed and all recovered embryos placed in vitro culture. |
Inquire | |
| Cryopreserved embryo recovery and transfer |
One straw thawed and embryos transferred into up to 2 recipients. |
Inquire | |
| Embryo cryopreservation |
Embryos collected from up to 15 females per session and cryopreserved. |
Inquire | |
| Sperm cryopreservation |
Sperms collected from up to 2 males per session and cryopreserved. Includes QC thawing of one straw to evaluate sperm quality. Includes 3 years of storage. |
Inquire | |
| ► IVF (1) | |||
| Name | Description | Price | |
| In vitro fertilization with cryopreserved sperms |
Oocytes from 5 donor C57BL females will be fertilized with fresh sperms of investigator's choice. Transfer fertilized embryos into recipient females. |
Inquire | |
| ► Injection Services (4) | |||
| Name | Description | Price | |
| Blastocyst injection (129 ES into C57BL/6J) Basic Service |
25 blastocysts injected and transferred into up to 3 recipients. |
Inquire | |
| Blastocyst injection (129 ES into C57BL/6J) Guaranteed Service |
25 blastocysts injected and transferred into up to 3 recipients. Two >50% chimeras are guaranteed for guaranteed service. |
Inquire | |
| ES cell tetraploid injection Basic Service |
> 50 2‐cell embryos per session will be used for fusion experiment, and resulting tetraploid blastocysts will be injected with ES cells |
Inquire | |
| Pronuclear injection (B6D2F1 hybrid) Guaranteed Service |
>120 eggs injected and transferred into up to 4 recipients. Two founders are guaranteed. |
Inquire | |
| ► Targeting Services (4) | |||
| Name | Description | Price | |
| ES cell derivation |
GTSR will start with up to 48 blastocysts per session. |
Inquire | |
| ES cell targeting |
Up to 192 ES clones provided to investigator for confirming HR |
Inquire | |
| Expanding targeted ES cell clones for injection |
One clone will be expanded for injection at a time. |
Inquire | |
| Expanding targeted ES cell clones for storage |
Up to 5 clones will be expanded and cryopreserved. |
Inquire | |
| ► Transfer Services (3) | |||
| Name | Description | Price | |
| Oviduct transfer |
Transfer up to 20 embryos to oviducts of 1 recipient |
Inquire | |
| SPF Rederivation |
Collect embryos from up to 10 donor females per session and transfer the embryos up to 3 SPF recipients. |
Inquire | |
| Uterus transfer |
Transfer up to 20 embryos to uteri of 1 recipient |
Inquire | |
